In our recent manuscript (https://www.biorxiv.org/content/10.1101/557082v1) we introduce MINUTE-ChIP, a quantitative multiplexed ChIP method based on Mint-ChIP by Brad Bernstein’s lab (van Galen et al., 2016; PMID: 26687680).
Why doing multiplexed quantitative ChIP? Multiplexing speaks for itself – many experiments all in one tube. But let’s talk about the need for quantitative ChIP. Here is a boat with an observer. A volcano island rises high above sea level.
Here is the boat again. For the observer, the peak now appears pretty small.
But we as an outside observer can see that it is not the volcano height that changed, it is the sea level that rose up to the peak.
Traditional ChIP normalisation assumes a constant background both on the technical and the biological level. Just like the observer in the boat who does not know the change in sea level, the method is blind to global alterations in histone modification levels.
Read the fill story at https://www.biorxiv.org/content/10.1101/557082v1