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PiggyBac VECTORS FOR TRANSIENT OR STABLE AMBER SUPPRESSION
Find our most useful plasmids on Addgene:
Further plasmids are available upon request.
Sequences of most current published and unpublished amber suppression plasmids can be found here:
The PiggyBac vector series for highly efficient amber suppression in transient or stable mammalian expression systems developed by Simon Elsässer and Jason Chin is available from the MRC Laboratory of Molecular Biology, subject to a Material Transfer Agreement. For inquiries please contact Fiona Townsley (fmt at mrc.lmb.cam.ac.uk). A detailed description of the vectors and procedure for generating stable cell lines can be found in the associated publications:
Genetic code expansion in stable cell lines enables encoded chromatin modification, Nature Methods (2016), doi:10.1038/NMETH.3701
Photoactivation of mutant isocitrate dehydrogenase 2 reveals rapid cancer-associated metabolic and epigenetic changes, JACS (2016)
Derivates of the vectors have also been published in the following papers, highlighting the versatility of the plasmid system:
Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1, JACS (2015), http://pubs.acs.org/doi/abs/10.1021/ja5069728
Genetic Code Expansion Enables Live-Cell and Super-Resolution Imaging of Site-Specifically Labeled Cellular Proteins, JACS (2015), http://pubs.acs.org/doi/abs/10.1021/ja512838z
Selective, rapid and optically switchable regulation of protein function in live mammalian cells, Nature Chemistry (2015), http://www.nature.com/nchem/journal/vaop/ncurrent/full/nchem.2253.html